Journal: Frontiers in Bioengineering and Biotechnology
Article Title: A novel cryoprecipitate-enriched PRP (Cryo-PRP) gel with enhanced mechanical strength and regenerative capacity accelerates enterocutaneous fistula healing
doi: 10.3389/fbioe.2025.1668608
Figure Lengend Snippet: Immunofluorescence and immunohistochemical analysis in fistula tissues. (A) Representative immunofluorescence staining of CD34 (red) and DAPI (blue) in fistula tissues from Control, PRP, and Cryo-PRP groups. (B) Quantitative analysis of CD34 expression using integrated optical density (IOD/Area). (C) Representative images of immunohistochemical staining for α-SMA, CD31, VEGF, PCNA, and TNF-α in the Control, PRP, and Cryo-PRP groups are shown (original magnification: ×400). Expression of α-SMA, CD31, VEGF, and PCNA was markedly increased in the PRP and Cryo-PRP groups compared to Control, with the highest levels observed in the Cryo-PRP group. In contrast, TNF-α expression was significantly reduced following PRP and Cryo-PRP treatment, with minimal staining in the Cryo-PRP group. (D) Quantitative analysis of immunohistochemical staining using integrated optical density per area (IOD/Area × 10 6 ). Data are presented as mean ± SD. * P < 0.05 vs. Control; *** P < 0.001 vs. Control.
Article Snippet: Subsequently, the sections were incubated overnight at 4 °C with rabbit anti-CD34 antibody (BIOSS, bs-064R; 1:100 dilution).
Techniques: Immunofluorescence, Immunohistochemical staining, Staining, Control, Expressing