rabbit anti cd34  (Bioss)

Journal: Journal of Traditional and Complementary Medicine

Article Title: Plasma-derived exosomal miR-144-3p targeting IL-1β is involved in pressure pain threshold regulation at PC6 acupoint in myocardial ischemia rats

doi: 10.1016/j.jtcme.2025.02.001

Figure Lengend Snippet: Exosomal miR-144-3p involved in PPT regulation and MCD in response to neurogenic inflammation and peripheral nociceptor hypersensitivity. ( A ) Immunohistochemical staining images showing colocalization of CD34 (MCs; red) and PGP9.5 (axons; green). Cell nuclei were stained with DAPI (blue). ( B ) Quantitative analyses of the colocalization of CD34 (MCs) and PGP9.5 (axons) (n = 3 per group). ( C ) The ratio of the number of cells expressing both CD34 (MCs) and PGP9.5 (axons) to the number of CD34 + MCs (n = 3 per group). All data are shown as the mean ± SD, ∗∗ P < 0.01, ## P < 0.01.

Article Snippet: The sections were incubated with primary antibodies including a rabbit polyclonal anti-CD34 antibody (Affinity Biosciences, Cincinnati, Ohio, USA) and rabbit polyclonal anti-Protein gene product 9.5 (PGP9.5) antibody (Affinity Biosciences, Cincinnati, Ohio, USA).

Techniques: Immunohistochemical staining, Staining, Expressing

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: A novel cryoprecipitate-enriched PRP (Cryo-PRP) gel with enhanced mechanical strength and regenerative capacity accelerates enterocutaneous fistula healing

doi: 10.3389/fbioe.2025.1668608

Figure Lengend Snippet: Immunofluorescence and immunohistochemical analysis in fistula tissues. (A) Representative immunofluorescence staining of CD34 (red) and DAPI (blue) in fistula tissues from Control, PRP, and Cryo-PRP groups. (B) Quantitative analysis of CD34 expression using integrated optical density (IOD/Area). (C) Representative images of immunohistochemical staining for α-SMA, CD31, VEGF, PCNA, and TNF-α in the Control, PRP, and Cryo-PRP groups are shown (original magnification: ×400). Expression of α-SMA, CD31, VEGF, and PCNA was markedly increased in the PRP and Cryo-PRP groups compared to Control, with the highest levels observed in the Cryo-PRP group. In contrast, TNF-α expression was significantly reduced following PRP and Cryo-PRP treatment, with minimal staining in the Cryo-PRP group. (D) Quantitative analysis of immunohistochemical staining using integrated optical density per area (IOD/Area × 10 6 ). Data are presented as mean ± SD. * P < 0.05 vs. Control; *** P < 0.001 vs. Control.

Article Snippet: Subsequently, the sections were incubated overnight at 4 °C with rabbit anti-CD34 antibody (BIOSS, bs-064R; 1:100 dilution).

Techniques: Immunofluorescence, Immunohistochemical staining, Staining, Control, Expressing